Phenylmethanesulfonyl fluoride

Synonym
Product Category
Product Code
CAS Number
MDL Number
Pubchem SID
Reaxys RN
α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, NSC 88499
Fluorinated Building Blocks, Probe Building Blocks
KUMI1F25
329-98-6
MFCD00007424
24898863
2088311
image-155171-Trimethyl(trifluoromethyl)silane_reagent.png
Chemical Name
Molecular Formula
Molecular Weight
Assay(Analysis Method)
Appearance
Melting Point
Packaging
Storage
Phenylmethanesulfonyl fluoride
C7H7FO2S
174.19
≥98.0%(GC)
White to Pale yellow Solid
92°C
5g,25g,50g,100g and 500g
Store away from strong oxidizing agents, amines, bases, water/moisture
Phenylmethylsulfonyl fluoride (PMSF) has found widespread practical use as general, irreversible inhibitors of proteolytic degradation. Its applications include but not limited to: 1) Used as a nonspecific, irreversible inhibitor of serine proteases and other enzymes, including acetylcholinesterase, palmityl coenzyme A deacylase, arylsulfatase A, chymotrypsin, and trypsin. 2) Used in protein solublization studies in order to deactivate proteases from digesting proteins of interest after cell lysis. 3) It can be used as a chemical label to identify essential active site serines in an enzyme,Because PMSF specifically sulfonylates the hydroxal groups of active site serine residues of enzymes. 4) known to alter the actions of anandamide by blocking its metabolism and can produce cannabinoid effects in mice, including antinociception, hypothermia, and immobility with ED50 values of 86, 224, and 206 mg/kg, respectively. 5) It is a widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB1 receptor activity.
Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers. [1]
Phenylmethylsulfonyl fluoride (PMSF) given systemically produces naloxone-reversible analgesia and potentiates effects of beta-endorphin given centrally. [2]
Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride. [3]
References:
[1] G. T. James, Analytical Biochem., 1978, 86(2), pp 574-579.
[2] C. Pinsky, A. K. Dua, F. S. Labella, Life Sci., 1982, 31(12-13), pp 1193-1196.
[3] D. Krant, H. Goff, R. K. Pai, N. A. Hosea, I. Silman, J. L. Sussman, P. Taylor, J. G. Voet, Mol. Pharmacol., 2000, 57(6), pp 1243-1248.
Signal Word
Hazard Statements
Precautionary Statements
HS Code
RIDADR
Legal Information
Danger
H301,H314
P280,P301+P310+P330,P303+P361+P353,P304+P340+P310,P305+P351+P338
2904.10.3700
UN 2928 8(6.1) / PG II

1. Product Specification
2. Safety Data Sheet
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