Store away from strong oxidizing agents, amines, bases, water/moisture
Phenylmethylsulfonyl fluoride (PMSF) has found widespread practical use as general, irreversible inhibitors of proteolytic degradation. Its applications include but not limited to: 1) Used as a nonspecific, irreversible inhibitor of serine proteases and other enzymes, including acetylcholinesterase, palmityl coenzyme A deacylase, arylsulfatase A, chymotrypsin, and trypsin. 2) Used in protein solublization studies in order to deactivate proteases from digesting proteins of interest after cell lysis. 3) It can be used as a chemical label to identify essential active site serines in an enzyme,Because PMSF specifically sulfonylates the hydroxal groups of active site serine residues of enzymes. 4) known to alter the actions of anandamide by blocking its metabolism and can produce cannabinoid effects in mice, including antinociception, hypothermia, and immobility with ED50 values of 86, 224, and 206 mg/kg, respectively. 5) It is a widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB1 receptor activity.
Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers. 
Phenylmethylsulfonyl fluoride (PMSF) given systemically produces naloxone-reversible analgesia and potentiates effects of beta-endorphin given centrally. 
Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride. 
 G. T. James, Analytical Biochem., 1978, 86(2), pp 574-579.
 C. Pinsky, A. K. Dua, F. S. Labella, Life Sci., 1982, 31(12-13), pp 1193-1196.
 D. Krant, H. Goff, R. K. Pai, N. A. Hosea, I. Silman, J. L. Sussman, P. Taylor, J. G. Voet, Mol. Pharmacol., 2000, 57(6), pp 1243-1248.