2-(3-But-3-ynyl-3H-diazirin-3-yl)-ethanol is a trifunctional building block for chemical probe synthesis, typically employed for photoaffinity labeling in chemical biology and medicinal chemistry. It contains a light-activated diazirine (photoreactive group), terminal alkyne tag (clickable handle), and hydroxyl synthetic handle (connectivity group). When appended to a ligand or pharmacophore through its hydroxyl linker, this probe building block allows for UV light-induced covalent modification of a biological target with potential for downstream applications via the alkyne tag.
Tandem photoaffinity labeling–bioorthogonal conjugation in medicinal chemistry.
Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. 
Reversible Inhibitors Arrest ClpP in a Defined Conformational State that Can Be Revoked by ClpX Association. 
Fluorescent Probes for Single-Step Detection and Proteomic Profiling of Histone Deacetylases. 
In Situ Proteome Profiling and Bioimaging Applications of Small-Molecule Affinity-Based Probes Derived From DOT1L Inhibitors.